Liver lobe and strain differences in the activity of murine cytochrome P450 enzymes

For toxico- and pharmacokinetic studies, use of liver-based microsomes, including those of mice, is state-of-the-art to study CYP-dependent metabolism. However, reproducibility and interpretation of these data is still very variable, partly because current testing guidelines do not cover details on organ sampling and potential liver lobe differences. The study indicates that in mice CYP activity and gene expression are strain dependent and may vary highly between liver lobes.

The article was published in the scientific journal "Toxicology".

Liver lobe and strain differences in the activity of murine cytochrome P450 enzymes
Juliane Rudeck, Bettina Bert, Philip Marx-Stoelting, Gilbert Schönfelder, Silvia Vog

Toxicology
Vol 404-405
1 July 2018, Pages 76-85
DOI: 10.1016/j.tox.2018.06.001